플라스미드 DNA로 대장균 형질전환시 회복단계의 필요여부에 대한 연구
Recovery step may be ommitted in transformation method
김미광,
소속 상세정보
김미광 ( ) - 조선대학교 치과대학 구강생화학교실 및 구강생물학 연구소
KMID : 0353320020260020297
Abstract
We previously introduced the direct transformation method, direct plating of transformation mixture onto LB agar plate after immediately mixing of competent cells and pUC19 plasmid DNA. In that report, the difference of transformation efficiency according to the recovery time of transformed E. coli with pUC19 was not determined. In this study, to completely establish the direct transformation method, transformation efficiency according to the recovery time of transformed E. coli was determined. Our data reveal that the transformation efficiency was increased the recovery time after heat shock. However, this result come from the mitosis of transformed E. coli with pUC19. Our data indicate recovery step may be omitted in transformation experiment.
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